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Full reconstitution guide with interactive dose calculator included with every order.
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Research Protocol Guide

Dosing &
Reconstitution

Everything you need to prepare, dose, and administer your Nexagen peptides correctly. Read this once before you start. It'll save you from making the most common mistakes.

⚠️ Research Use Only All Nexagen products are sold strictly for research purposes. This guide is for informational use only and does not constitute medical advice. Not for human consumption. Always consult applicable research documentation before beginning any protocol.
01
What You Need
Required supplies before you start
✓ The Nexagen Reconstitution Starter Kit includes everything below Available in the Accessories section of our catalog. Grab one and you're ready to go.
💧

Bacteriostatic Water (BAC Water)

This is NOT regular water. Bacteriostatic water contains 0.9% benzyl alcohol which prevents bacterial growth after you open the vial. You must use BAC water — not sterile water, not tap water, not saline. Available in our accessories section for $12.

One 10ml BAC water vial is typically enough to reconstitute 2 to 4 peptide vials depending on how much water you add per vial.
💉

Insulin Syringes — 29G or 31G

Use standard U-100 insulin syringes with a 1ml capacity. The gauge (29G or 31G) refers to needle thickness. 31G is thinner and more comfortable. 29G is slightly easier to draw with. Both work perfectly for subcutaneous peptide research.

Never reuse syringes. Use one per draw for reconstitution and a fresh one for each administration.
🧹

Alcohol Swabs

70% isopropyl alcohol swabs. Use one to sterilize the rubber top of every vial before inserting any needle. Use a second one to clean the injection site. Let it dry completely before injecting — wet alcohol stings and can carry contamination into the puncture.

🗑️

Sharps Disposal Container

Federal and state regulations require proper sharps disposal. Never recap needles and never throw them in regular trash. A 1-quart sharps container handles months of protocols for most research applications.

02
Reconstitution
How to mix your freeze-dried peptide with BAC water

Nexagen peptides arrive as a lyophilized (freeze-dried) powder. Before use they need to be reconstituted — mixed with bacteriostatic water to create a solution. This process is simple and takes about 2 minutes.

💡 How much BAC water to add? The most common amount is 1ml to 2ml per vial. Adding 1ml creates a more concentrated solution (larger doses per unit volume). Adding 2ml creates a more dilute solution (easier to measure small doses). Use our calculator below to find the exact right amount for your protocol.
1

Wash Your Hands

Thoroughly wash both hands with soap and water for at least 20 seconds. This is the most overlooked step and the most important one for preventing contamination.

2

Swab Both Vial Tops

Use a fresh alcohol swab on the rubber stopper of your peptide vial and on your BAC water vial. Wipe in one direction — do not scrub back and forth. Let both dry completely for 10 to 15 seconds before proceeding.

3

Draw BAC Water into Your Syringe

Insert the needle into the BAC water vial and draw up the amount of water your protocol requires (typically 1ml to 2ml). Pull the plunger slowly and steadily. Remove the needle from the BAC water vial.

On a U-100 insulin syringe: 1ml = 100 units on the barrel. Half a ml = 50 units. 0.1ml = 10 units.
4

Inject Water into Peptide Vial — Slowly

Insert the needle into your peptide vial at a slight angle so the water runs down the inside of the glass wall rather than spraying directly onto the powder. Push the plunger slowly and steadily. Never inject fast — this can degrade the peptide.

If you see the powder clumping or you're forcing it — slow down. The water should barely disturb the powder at first.
5

Do NOT Shake — Gently Roll or Swirl

After adding the water, gently swirl the vial between your palms or slowly roll it back and forth. The powder will dissolve on its own within 30 to 60 seconds. Never shake the vial — shaking creates bubbles and can break down the peptide bonds.

6

Inspect the Solution

Your reconstituted peptide should be clear and colorless (most peptides) or very slightly yellow (some like BPC-157 and TB-500 are naturally slightly colored). If you see cloudiness, floating particles, or unusual color — do not use it.

A small amount of bubbles is normal after swirling. Let the vial sit for 2 to 3 minutes and they will settle on their own.
7

Label and Refrigerate Immediately

Write the date of reconstitution on your vial with a marker or label. Place immediately in the refrigerator (2°C to 8°C / 36°F to 46°F). A reconstituted peptide stored properly in the refrigerator is typically stable for 28 to 30 days.

⚠️ Critical: Never Freeze a Reconstituted Peptide Freeze-dried powder can be stored frozen. Once mixed with BAC water, freezing destroys the peptide. Always keep reconstituted peptides refrigerated — never the freezer.
03
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04
Injection Guide
Subcutaneous administration — step by step

All Nexagen peptides are administered subcutaneously (SubQ) — meaning just under the skin into the fatty tissue. This is different from intramuscular (IM) injection. SubQ is shallow, comfortable, and easy to self-administer.

📍

Best Injection Sites

Rotate between sites to prevent tissue buildup. The abdomen (around but not on the navel) is the most popular site — plenty of fat tissue and easy to access. Alternate sides each injection.

Abdomen ✓ Best Thigh ✓ Good Upper arm ✓ OK Lower back ✓ OK
1

Draw Your Dose

Swab the rubber top of your reconstituted peptide vial with an alcohol swab. Let it dry. Insert the needle and draw up your calculated dose. Remove any air bubbles by flicking the syringe gently and pushing the plunger up until a tiny drop appears at the needle tip.

2

Choose and Clean Your Injection Site

Select a site with adequate subcutaneous fat — stomach, thigh, or upper arm. Wipe the area with an alcohol swab and let it dry completely. A circle about the size of a golf ball defines your injection zone.

Avoid injecting into the same exact spot two days in a row. Rotate around the zone to prevent irritation.
3

Pinch and Insert at 45°

With your non-dominant hand, gently pinch a fold of skin about 1 inch wide. Hold the syringe like a dart in your dominant hand. Insert the needle at a 45-degree angle (or 90° if you have significant fat tissue) with a quick, confident motion. Hesitation causes more discomfort than speed.

4

Inject Slowly

Release the skin pinch. Push the plunger down slowly and steadily over 5 to 10 seconds. Do not rush. A slow steady injection is more comfortable and prevents the solution from backing up around the needle.

5

Withdraw and Apply Light Pressure

Pull the needle out at the same angle you inserted it. Apply light pressure with a clean alcohol swab or cotton ball for 10 to 15 seconds. Do not rub — this can cause the solution to disperse unevenly or cause irritation.

6

Dispose of the Needle Properly

Place the used needle immediately into your sharps disposal container. Never recap, never reuse, never throw in regular trash. A full sharps container can be dropped off at most pharmacies or medical waste collection sites.

✓ Do This

  • Always swab vial tops before inserting any needle
  • Let alcohol dry completely before injecting
  • Rotate injection sites every time
  • Inject slowly and steadily
  • Use a fresh needle for each injection
  • Dispose properly in sharps container
  • Keep reconstituted peptides refrigerated
  • Check your solution is clear before drawing

✗ Never Do This

  • Shake the vial — ever
  • Inject into a vein or muscle (SubQ only)
  • Use regular water instead of BAC water
  • Reuse needles or syringes
  • Freeze reconstituted peptides
  • Inject a cloudy or particulate solution
  • Skip the alcohol swab on vial tops
  • Leave reconstituted peptides at room temperature
05
Storage Guide
Keep your peptides potent from delivery to last dose
Condition Lyophilized (Dry Powder) Reconstituted (Mixed) Status
Refrigerator
2°C to 8°C / 36°F to 46°F
6 to 12 months 28 to 30 days Optimal
Freezer
-20°C / -4°F
12 to 24 months ❌ Never freeze Powder Only
Room Temperature
20°C to 25°C / 68°F to 77°F
2 to 4 weeks (avoid) 24 to 48 hours max Avoid
Travel / Transport
With cold pack
Up to 24 hours fine Up to 24 hours with cold pack Use Cold Pack
Direct Sunlight / Heat
> 30°C / 86°F
Degrades rapidly Degrades rapidly Never
⚠️ Shipping Note — 5 to 14 Business Days Your Nexagen peptides ship as dry lyophilized powder which is stable at room temperature for short transit periods. Upon arrival, inspect your package and store powder vials in the refrigerator or freezer immediately. Only reconstitute what you plan to use within 30 days.
💡 Travel Tip The Nexagen Travel Cold Pack keeps your peptides at proper temperature for up to 24 hours — ideal for research travel. For longer trips, dry powder vials can be transported at room temperature for a few days without significant degradation.
06
GLP-1 Dosing Reference
Semaglutide · Tirzepatide · Retatrutide titration guidance

GLP-1 class peptides (Semaglutide, Tirzepatide, Retatrutide) follow a titration protocol — starting low and increasing gradually over time. This is essential for managing tolerability. Jumping to a high dose immediately causes unnecessary nausea and discomfort.

⚠️ Start Low — Always Titrate Up The single biggest mistake with GLP-1 protocols is starting at too high a dose. The research strongly supports beginning at the lowest dose and increasing only after tolerating the previous dose for at least 4 weeks.
📅

Weeks 1 to 4 — Start Phase

Begin at the lowest available dose. For Semaglutide this is typically 0.25mg weekly. For Tirzepatide this is typically 2.5mg weekly. For Retatrutide this is typically 1mg to 2mg weekly. Inject once per week on the same day each week.

Pick a consistent day — Sunday, for example — and always inject on that day. Consistency is critical for GLP-1 protocols.
📅

Weeks 5 to 8 — First Increase

If the starting dose is well tolerated (minimal nausea, manageable appetite suppression), increase to the next dose increment. Typical increases: Semaglutide 0.5mg, Tirzepatide 5mg, Retatrutide 2mg to 4mg weekly.

📅

Weeks 9 and Beyond — Maintenance

Continue increasing every 4 weeks until you reach your target maintenance dose or the dose where you experience optimal results with acceptable tolerability. There is no requirement to reach a maximum dose — use the dose that works for your research protocol.

Many research subjects find their optimal dose is lower than the maximum. More is not always better with GLP-1 compounds.
✓ Managing Nausea Mild nausea is common when starting or increasing GLP-1 doses. It typically passes within a few days. Eating smaller meals, avoiding fatty or spicy foods, and staying hydrated helps significantly. If nausea is severe or persistent — stay at your current dose for another 2 weeks before increasing.
💡 Once Weekly vs. More Frequent Dosing Semaglutide, Tirzepatide, and Retatrutide are all designed for once-weekly subcutaneous injection based on their pharmacokinetic profiles. Unlike shorter peptides that may be dosed daily or multiple times per week — these compounds have long half-lives that make once weekly the standard protocol.
07
Frequently Asked Questions
The most common reconstitution and dosing questions
What happens if I accidentally shake my peptide vial?
A brief accidental shake is unlikely to completely destroy the peptide but it's not ideal. Peptide bonds can be damaged by aggressive agitation. If you accidentally shook it briefly, let it settle for a few minutes and inspect the solution. If it looks clear with no particles — it's likely fine. If you vigorously shook it for an extended period, the peptide may be degraded. When in doubt, reconstitute a fresh vial.
Can I use sterile water instead of bacteriostatic water?
Technically you can use sterile water for reconstitution — but you should use the entire vial within 24 hours because sterile water has no preservative to prevent bacterial growth. Bacteriostatic water contains 0.9% benzyl alcohol which keeps your reconstituted peptide safe in the refrigerator for up to 30 days. For any multi-dose protocol, BAC water is the correct choice.
My solution looks slightly yellow — is that normal?
Yes. Several peptides have a naturally slight yellow or off-white tint when reconstituted — particularly BPC-157, TB-500, and some GH peptides. This is completely normal and does not indicate degradation or contamination. What you want to avoid is cloudiness, floating particles, or an unusual color that wasn't present when you first mixed it. A clear or very slightly tinted solution is correct.
How do I know if my peptide has gone bad?
Signs that a reconstituted peptide may be degraded or contaminated: cloudiness or haziness that wasn't there initially, floating particles or debris, unusual smell, or a significant color change. If a vial is more than 30 days old since reconstitution — discard it. Dry powder that has been stored improperly (exposed to heat, light, or moisture) may also be degraded. When in doubt, don't use it.
I drew too much solution into my syringe — what do I do?
If the needle has not touched anything non-sterile, you can push the excess back into the vial. Insert the needle back into the vial through the rubber stopper and slowly push the plunger until you reach your correct unit mark. If the needle has touched anything — discard it and use a fresh syringe to draw the correct amount.
How many units do I draw for a 250mcg (0.25mg) dose from a 5mg vial reconstituted with 1ml of BAC water?
Easy one to walk through: Your vial has 5mg in 1ml = concentration of 5mg per ml = 5000mcg per ml. Your dose is 250mcg. Divide: 250 ÷ 5000 = 0.05ml. On a U-100 syringe that is 5 units. Draw to the 5 unit line. You can verify this with our calculator above — enter vial size 5mg, BAC water 1ml, dose 250mcg.
Is subcutaneous injection painful?
Most research subjects report minimal to no pain with subcutaneous injections using a 31G needle — far less than a typical blood draw. The key factors are: letting the alcohol dry completely before injecting (wet alcohol burns), using a fresh needle every time (dull needles hurt), inserting quickly and confidently rather than hesitating, and injecting the solution slowly. The abdomen generally has less pain sensitivity than other sites for most people.
Can I mix two peptides in the same syringe?
In some research protocols, peptides are combined in the same syringe for administration — particularly GH stacks like CJC-1295 + Ipamorelin. The pre-combined products in our catalog (BPC+TB combo, Klow stack) are already blended at the factory level. For home mixing: draw one peptide into the syringe, then draw the second. Do not transfer back. Keep amounts small and administer immediately — don't mix and store. Consult your specific protocol documentation before combining compounds.
My powder didn't dissolve completely — what do I do?
Some peptides take longer to dissolve than others — particularly larger compounds like TB-500. If there's still undissolved powder after gentle swirling: let the vial sit in the refrigerator for 15 to 30 minutes and then gently roll it again. Do not heat it or shake it. Most stubborn powders will dissolve completely with a little more time. If particles remain after 30 minutes of rest — the peptide may have been damaged before or during shipping. Contact Nexagen support.
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